Anticorps Recombinant de lapin anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

PE-Cyanine7 Fluorescent Dye

CloneNo.

M5/114.15.2

N° de cat : PY7-FcA65663

Synonymes

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PY7-FcA65663

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Mouse CD3 (17A2), and 0.25 ug PE-Cyanine7 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (PY7-FcA65663, Clone: M5/114.15.2) or FcZero-rAb™ PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-FcA98136, Clone: 240953C9). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PY7-FcA65663

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE-Cyanine7 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (PY7-FcA65663, Clone: M5/114.15.2) (red) or FcZero-rAb™ PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-FcA98136, Clone: 240953C9) (blue). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PY7-FcA65663 cible MHC Class II (I-A/I-E) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: MHC Class II
• Identification du gène (NCBI): 14960
• Conjugaison: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

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