Anticorps Polyclonal de lapin anti-MIB1

• Phare
• Validé par KD/KO

MIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 11893-1-AP

Synonymes

DAPK interacting protein 1, DAPK-interacting protein 1, DIP 1, DIP1, DIP-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 11893-1-AP

  WB result of MIB1 antibody (11893-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIB1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11893-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11893-1-AP (MIB1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11893-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11893-1-AP (MIB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11893-1-AP

  IP result of anti-MIB1 (IP:11893-1-AP, 4ug; Detection:11893-1-AP 1:500) with K-562 cells lysate 3400ug.

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  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 11893-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIB1 antibody (11893-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11893-1-AP cible MIB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIB1 Protéine recombinante Ag2507
• Nom complet: mindbomb homolog 1 (Drosophila)
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC022403
• Symbole du gène: MIB1
• Identification du gène (NCBI): 57534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

Publications

Species	Application	Title
mouse,human	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	WB	Cancer Res; Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.; Authors - Yoshihiko Tasaki
human	WB	Cancer Lett; LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer; Authors - Hansen Lin
mouse	WB	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated
human	WB	Cell Death Dis; hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.; Authors - Xinxing Wan
human	WB	MedComm (2020); Sanggenon C inhibits cell proliferation and induces apoptosis by regulating the MIB1/DAPK1 axis in glioblastoma; Authors - Hongbo Chang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (04-22-2021)

Good antibody, clear band seen in parental vs MIB1 knockout A549 cell

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549

MIB1 Antibody Western Blot validation (1:1000 dilution) in A549 (Cat no:11893-1-AP)

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