Anticorps Monoclonal anti-MICA

MICA Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E2C8

N° de cat : 66384-1-PBS

Synonymes

1E2C8, MIC A, MIC-A

---

Galerie de données de validation

• 

  WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66384-1-Ig (same clone as 66384-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66384-1-Ig (same clone as 66384-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66384-1-Ig (same clone as 66384-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66384-1-Ig (same clone as 66384-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66384-1-Ig (same clone as 66384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66384-1-Ig (same clone as 66384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66384-1-PBS cible MICA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MICA Protéine recombinante Ag3298
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Poids moléculaire observé: 43-50 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).

{{ptg:RelatedPrimaryAntibodies}}