Anticorps Recombinant de lapin anti-MICA

MICA Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240735D8

N° de cat : 83725-3-PBS

Synonymes

MIC A, MIC-A

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00686-1

  Sandwich ELISA standard curve of MP00686-1, Human MICA Recombinant Matched Antibody Pair - PBS only. 83725-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1176. 83725-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

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  Sample test of MP00686-1

  HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 70.4 pg/mL.
  HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 285.9 pg/mL.

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  Cytometric bead array standard curve of MP00686-1

  Cytometric bead array standard curve of MP00686-1, MICA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83725-3-PBS. Detection antibody: 83725-1-PBS. Standard: Eg1176. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83725-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 83725-3-PBS against Human MICA were performed. The affinity constant is 0.819 nM.

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Informations sur le produit

83725-3-PBS cible MICA dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MICA Protéine recombinante Eg1176
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 43 kDa
• Numéro d’acquisition GenBank: AAH16929.1
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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