Anticorps Recombinant de lapin anti-MICA/MICB

MICA/MICB Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240735D9

N° de cat : 83725-5-RR

Synonymes

MICB, MICA, PERB11.1, MIC A, 240735D9

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Galerie de données de validation

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  WB analysis using 83725-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83725-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83725-5-RR cible MICA/MICB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: AAH16929.1
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

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