Anticorps Recombinant de lapin anti-MICA

MICA Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242004C2

N° de cat : 84850-5-RR

Synonymes

MIC-A, MIC A, 242004C2

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Galerie de données de validation

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  WB analysis of HeLa using 84850-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84850-5-RR (MICA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84850-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84850-5-RR (MICA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84850-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84850-5-RR against Human MICA were performed. The affinity constant is 7.61 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84850-5-RR cible MICA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).

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