Anticorps Recombinant de lapin anti-MICA/MICB

MICA/MICB Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

6D4

N° de cat : FITC-65589

Synonymes

MICB, MICA, 6D4, MIC A, MIC-A

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Galerie de données de validation

• 

  FC experiment of HeLa using FITC-65589

  1x10^6 HeLa cells were surface stained with 5 ul FITC Plus Anti-Human MICA/MICB (6D4) Mouse IgG2a RecAb (FITC-65589, Clone: 6D4) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65589 cible MICA/MICB dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules C1R transfectées exprimant MICA
• Nom complet: MHC class I polypeptide-related sequence A
• Masse moléculaire calculée: 383 aa, 43 kDa
• Numéro d’acquisition GenBank: BC016929
• Symbole du gène: MICA
• Identification du gène (NCBI): 100507436
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

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