Anticorps Polyclonal de lapin anti-MIDN

• Phare
• Validé par KD/KO

MIDN Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18939-1-AP

Synonymes

Midnolin

---

Galerie de données de validation

• 

  WB analysis using 18939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules PC-12, cellules BxPC-3, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

18939-1-AP cible MIDN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIDN Protéine recombinante Ag13492
• Nom complet: midnolin
• Masse moléculaire calculée: 468 aa, 49 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC094778
• Symbole du gène: MIDN
• Identification du gène (NCBI): 90007
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.

Publications

Species	Application	Title
mouse	WB	Mol Cell Biol; CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers.; Authors - Sam Van de Velde
human	WB	Science; The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation; Authors - Xin Gu ; KO Validated
human	IHC,WB	Cancer Sci; Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling; Authors - Weixi Wang
human	WB,IF	Biomedicines; Comprehensive Analysis Reveals Midnolin as a Potential Prognostic, Therapeutic, and Immunological Cancer Biomarker; Authors - Xin-Guo Zhang ; KD Validated
mouse	WB	J Cell Mol Med; Midnolin Correlates With Anti-Tumour Immunity and Promotes Liver Cancer Progression Through β-Catenin; Authors - Shaobo Huang
pig	WB	J Virol; Midnolin inhibits coronavirus proliferation by degrading viral proteins; Authors - Yahe Wang ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}