Anticorps Polyclonal de lapin anti-MIER1

MIER1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11452-1-PBS

Synonymes

MIER 1, MI ER1, hMi-er1, hMIER1, hMI ER1

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Galerie de données de validation

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  WB analysis of human testis using 11452-1-AP (same clone as 11452-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11452-1-AP (same clone as 11452-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11452-1-AP (same clone as 11452-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 11452-1-AP (same clone as 11452-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11452-1-AP (MIER1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 11452-1-AP (same clone as 11452-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MIER1 Polyclonal antibody (11452-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11452-1-PBS cible MIER1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIER1 Protéine recombinante Ag2010
• Nom complet: mesoderm induction early response 1 homolog (Xenopus laevis)
• Masse moléculaire calculée: 536 aa, 61 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC017423
• Symbole du gène: MIER1
• Identification du gène (NCBI): 57708
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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