Anticorps Recombinant de lapin anti-MIER2

MIER2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241458D2

N° de cat : 84216-1-PBS

Synonymes

241458D2, KIAA1193, Mesoderm induction early response protein 2, Mi-er2

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Galerie de données de validation

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  WB analysis using 84216-1-RR (same clone as 84216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84216-1-RR (same clone as 84216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01148-1

  Cytometric bead array standard curve of MP01148-1, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-2-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01148-2

  Cytometric bead array standard curve of MP01148-2, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-3-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84216-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84216-1-PBS cible MIER2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MIER2 Protéine recombinante Ag11491
• Nom complet: mesoderm induction early response 1, family member 2
• Masse moléculaire calculée: 545 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC028203
• Symbole du gène: MIER2
• Identification du gène (NCBI): 54531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).

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