Anticorps Polyclonal de lapin anti-MIF4GD

MIF4GD Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24288-1-PBS

Synonymes

SLIP1, MIFD, MIF4G, hSLIP1, AD023

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Galerie de données de validation

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  WB analysis using 24288-1-AP (same clone as 24288-1-PBS)

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 24288-1-AP (MIF4GD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24288-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24288-1-AP (same clone as 24288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24288-1-AP (MIF4GD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24288-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24288-1-PBS cible MIF4GD dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIF4GD Protéine recombinante Ag17931
• Nom complet: MIF4G domain containing
• Masse moléculaire calculée: 256 aa, 29 kDa
• Poids moléculaire observé: ~25 kDa
• Numéro d’acquisition GenBank: BC033759
• Symbole du gène: MIF4GD
• Identification du gène (NCBI): 57409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MIF4GD (MIF4G domain containing), also known as MIFD, AD023, or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events.

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