Anticorps Polyclonal de lapin anti-MIG6; ERRFI1

• Phare
• Validé par KD/KO

MIG6; ERRFI1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11630-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 11630-1-AP (same clone as 11630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11630-1-AP (same clone as 11630-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MIG6; ERRFI1 antibody (11630-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11630-1-PBS cible MIG6; ERRFI1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIG6; ERRFI1 Protéine recombinante Ag2202
• Nom complet: ERBB receptor feedback inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC025337
• Symbole du gène: MIG6
• Identification du gène (NCBI): 54206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

{{ptg:RelatedPrimaryAntibodies}}