Anticorps Polyclonal de lapin anti-MIOS

MIOS Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20826-1-AP

Synonymes

GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

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Galerie de données de validation

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  WB analysis of K-562 using 20826-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 20826-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20826-1-AP

  IP result of anti-MIOS (IP:20826-1-AP, 4ug; Detection:20826-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules PC-3, tissu hépatique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

20826-1-AP cible MIOS dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIOS Protéine recombinante Ag14802
• Nom complet: missing oocyte, meiosis regulator, homolog (Drosophila)
• Masse moléculaire calculée: 875 aa, 99 kDa
• Poids moléculaire observé: 99 kDa
• Numéro d’acquisition GenBank: BC005883
• Symbole du gène: MIOS
• Identification du gène (NCBI): 54468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510

Publications

Species	Application	Title
pig	WB	Am J Physiol Endocrinol Metab; Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.; Authors - Marko Rudar
human	WB	JCI Insight; GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma; Authors - Jacqueline Morales ; KD Validated
human	WB	Nat Commun; Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer; Authors - Shankha S Chatterjee

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