Anticorps Recombinant de lapin anti-MIOS

MIOS Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240832B9

N° de cat : 83856-5-PBS

Synonymes

240832B9, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

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Galerie de données de validation

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  WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83856-5-RR (same clone as 83856-5-PBS)

  WB result of MIOS antibody (83856-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIOS transfected HeLa cells. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83856-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83856-5-RR against Human MIOS were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83856-5-PBS cible MIOS dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MIOS Protéine recombinante Ag15148
• Nom complet: missing oocyte, meiosis regulator, homolog (Drosophila)
• Masse moléculaire calculée: 875 aa, 99 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC005883
• Symbole du gène: MIOS
• Identification du gène (NCBI): 54468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510

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