Anticorps Recombinant de lapin anti-MIOS

MIOS Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240832B9

N° de cat : 83856-5-RR

Synonymes

240832B9, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

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Galerie de données de validation

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  WB analysis using 83856-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83856-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83856-5-RR

  WB result of MIOS antibody (83856-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIOS transfected HeLa cells.

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  Affinity and Kinetic Characterization of 83856-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83856-5-RR against Human MIOS were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83856-5-RR cible MIOS dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MIOS Protéine recombinante Ag15148
• Nom complet: missing oocyte, meiosis regulator, homolog (Drosophila)
• Masse moléculaire calculée: 875 aa, 99 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC005883
• Symbole du gène: MIOS
• Identification du gène (NCBI): 54468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510

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