Anticorps Polyclonal de lapin anti-CCL19/MIP-3 beta

CCL19/MIP-3 beta Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13397-1-PBS

Synonymes

CCL19, MIP-3 Beta, MIP-3-beta, Beta chemokine exodus 3, Beta-chemokine exodus-3

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Galerie de données de validation

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  WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13397-1-PBS cible CCL19/MIP-3 beta dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCL19/MIP-3 beta Protéine recombinante Ag4200
• Nom complet: chemokine (C-C motif) ligand 19
• Masse moléculaire calculée: 98 aa, 12 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC027968
• Symbole du gène: CCL19/MIP-3 beta
• Identification du gène (NCBI): 6363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

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