Anticorps Recombinant de lapin anti-MITF

MITF Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

240815E7

N° de cat : 83803-2-PBS

Synonymes

240815E7, bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor

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Galerie de données de validation

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  IF Staining of HepG2 using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant antibody (83803-2-RR, Clone:240815E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  ChIP experiment of K-562 using 83803-2-RR (same clone as 83803-2-PBS)

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MITF (83803-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83803-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83803-2-PBS against Human MITF was performed. The affinity constant is 3.09 nM.

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  Affinity and Kinetic Characterization of 83803-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83803-2-RR against Human MITF were performed. The affinity constant is 31.9 pM.

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Informations sur le produit

83803-2-PBS cible MITF dans les applications de IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MITF Protéine recombinante Ag3679
• Nom complet: microphthalmia-associated transcription factor
• Masse moléculaire calculée: 91 aa, 10 kDa, 59 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC012503
• Symbole du gène: MITF
• Identification du gène (NCBI): 4286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).

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