Anticorps Polyclonal de lapin anti-MIXL1

MIXL1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22772-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 22772-1-AP (same clone as 22772-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22772-1-PBS in a different storage buffer formulation.

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  WB analysis of rat pancreas using 22772-1-AP (same clone as 22772-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22772-1-PBS in a different storage buffer formulation.

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  WB analysis of H7ES using 22772-1-AP (same clone as 22772-1-PBS)

  H7ES cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22772-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 22772-1-AP (same clone as 22772-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 22772-1-AP (MIXL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22772-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22772-1-PBS cible MIXL1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIXL1 Protéine recombinante Ag18732
• Nom complet: Mix1 homeobox-like 1 (Xenopus laevis)
• Masse moléculaire calculée: 232 aa, 25 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC113441
• Symbole du gène: MIXL1
• Identification du gène (NCBI): 83881
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.

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