Anticorps Polyclonal de lapin anti-MKL1/MRTF-A

• Phare
• Validé par KD/KO

MKL1/MRTF-A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(39)

Avis (1)

N° de cat : 21166-1-AP

Synonymes

MKL1, KIAA1438, MAL, MKL/myocardin-like protein 1, MRTFA

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Galerie de données de validation

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  WB analysis using 21166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 21166-1-AP

  Immunofluorescent analysis of HeLa cells using 21166-1-AP (MKL1/MRTF-A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 18 publications below
• ChIP: See 5 publications below

Informations sur le produit

21166-1-AP cible MKL1/MRTF-A dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MKL1/MRTF-A Protéine recombinante Ag15240
• Nom complet: megakaryoblastic leukemia (translocation) 1
• Masse moléculaire calculée: 931 aa, 99 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC115039
• Symbole du gène: MKL1
• Identification du gène (NCBI): 57591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).

Publications

Species	Application	Title
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
mouse	IF	Nat Cell Biol; Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation; Authors - Miguel Bernabé-Rubio
	IF	J Cell Biol; The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression.; Authors - Jaime Fernández-Barrera
mouse	IF	Elife; Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.; Authors - Colin J Dinsmore
human	WB,IHC,IF,ChIP	Exp Mol Med; MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β; Authors - Fu Du ; KD Validated
human	WB	Oncotarget; The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells.; Authors - Hongpeng He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Qingxue (Verified Customer) (06-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs

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