Anticorps Recombinant de lapin anti-MKL1/MRTF-A

MKL1/MRTF-A Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242021B4

N° de cat : 84903-1-PBS

Synonymes

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

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Galerie de données de validation

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  WB analysis using 84903-1-RR (same clone as 84903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84903-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84903-1-PBS cible MKL1/MRTF-A dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MKL1/MRTF-A Protéine recombinante Ag15308
• Nom complet: megakaryoblastic leukemia (translocation) 1
• Masse moléculaire calculée: 931 aa, 99 kDa
• Poids moléculaire observé: 145-160 kDa
• Numéro d’acquisition GenBank: BC115039
• Symbole du gène: MKL1
• Identification du gène (NCBI): 57591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).

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