Anticorps Recombinant de lapin anti-MKL1/MRTF-A

MKL1/MRTF-A Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242021B4

N° de cat : 84903-1-RR

Synonymes

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

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Galerie de données de validation

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  WB analysis using 84903-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84903-1-RR cible MKL1/MRTF-A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MKL1/MRTF-A Protéine recombinante Ag15308
• Nom complet: megakaryoblastic leukemia (translocation) 1
• Masse moléculaire calculée: 931 aa, 99 kDa
• Poids moléculaire observé: 145-160 kDa
• Numéro d’acquisition GenBank: BC115039
• Symbole du gène: MKL1
• Identification du gène (NCBI): 57591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).

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