Anticorps Polyclonal de lapin anti-Muskelin

• Phare
• Validé par KD/KO

Muskelin Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 14735-1-AP

Synonymes

MKLN1, Muskelin, TWA2

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Galerie de données de validation

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  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14735-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14735-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14735-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 14735-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules Jurkat
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

14735-1-AP cible Muskelin dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Muskelin Protéine recombinante Ag6147
• Nom complet: muskelin 1, intracellular mediator containing kelch motifs
• Masse moléculaire calculée: 735 aa, 85 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC067825
• Symbole du gène: Muskelin
• Identification du gène (NCBI): 4289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IP,IF	Neuron; Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.; Authors - Frank F Heisler ; KO Validated
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB,IP	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated
human	WB	Cell Rep; mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase; Authors - Brittany Q Pham

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (12-31-2021)

This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and several others

Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T and several others (Cat no:14735-1-AP)

FH

H (Verified Customer) (04-05-2020)

Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14735-1-AP)

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