Anticorps Polyclonal de lapin anti-MKNK2

• Phare
• Validé par KD/KO

MKNK2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17354-1-PBS

Synonymes

MNK2, MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7

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Galerie de données de validation

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  WB analysis of mouse spleen using 17354-1-AP (same clone as 17354-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 17354-1-AP (same clone as 17354-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17354-1-AP (same clone as 17354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17354-1-AP (same clone as 17354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 17354-1-AP (same clone as 17354-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (17354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17354-1-PBS cible MKNK2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MKNK2 Protéine recombinante Ag10546
• Nom complet: MAP kinase interacting serine/threonine kinase 2
• Masse moléculaire calculée: 465 aa, 52 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC018345
• Symbole du gène: MKNK2
• Identification du gène (NCBI): 2872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.

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