Anticorps Recombinant de lapin anti-MKNK2

MKNK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240045B3

N° de cat : 83235-1-RR

Synonymes

MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1

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Galerie de données de validation

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  IF Staining of MCF-7 using 83235-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (83235-1-RR, Clone: 240045B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83235-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of MCF-7 using 83235-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83235-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83235-1-RR against Human MKNK2 were performed. The affinity constant is 75.1 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83235-1-RR cible MKNK2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MKNK2 Protéine recombinante Ag10546
• Nom complet: MAP kinase interacting serine/threonine kinase 2
• Masse moléculaire calculée: 465 aa, 52 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC018345
• Symbole du gène: MKNK2
• Identification du gène (NCBI): 2872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.

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