Anticorps Polyclonal de lapin anti-MKRN2

• Phare
• Validé par KD/KO

MKRN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12238-1-PBS

Synonymes

E3 ubiquitin-protein ligase makorin-2, EC:2.3.2.27, HSPC070, makorin ring finger protein 2, RING finger protein 62

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Galerie de données de validation

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  WB analysis using 12238-1-AP (same clone as 12238-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12238-1-AP (same clone as 12238-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 12238-1-AP (same clone as 12238-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12238-1-AP (same clone as 12238-1-PBS)

  IP result of anti-MKRN2 (IP:12238-1-AP, 4ug; Detection:12238-1-AP 1:2000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12238-1-PBS cible MKRN2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MKRN2 Protéine recombinante Ag2881
• Nom complet: makorin ring finger protein 2
• Masse moléculaire calculée: 416 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015715
• Symbole du gène: MKRN2
• Identification du gène (NCBI): 23609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.

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