Anticorps Polyclonal de lapin anti-Melan-A

Melan-A Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 18472-1-AP

Synonymes

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, MLANA, Protein Melan A

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Galerie de données de validation

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 40x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

18472-1-AP cible Melan-A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Melan-A Protéine recombinante Ag13346
• Nom complet: melan-A
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 13-20 kDa
• Numéro d’acquisition GenBank: BC014423
• Symbole du gène: MLANA
• Identification du gène (NCBI): 2315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)

Publications

Species	Application	Title
mouse	IHC	Int J Mol Sci; The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment; Authors - Karnoon Shamsoon
mouse	WB	Life Sci; Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells; Authors - Masaki Arioka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chromogenic Kit from Leica Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Melanoma Tuor

Melan-A Antibody Immunohistochemistry validation (1:500 dilution) in Melanoma Tuor (Cat no:18472-1-AP)