Anticorps Monoclonal anti-Melan-A

Melan-A Monoclonal Antibody for IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

4F10D6

N° de cat : 60348-1-Ig

Synonymes

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, Melan-A , MLANA, Protein Melan A

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Galerie de données de validation

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de mélanome malin humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

60348-1-Ig cible Melan-A dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Melan-A Protéine recombinante Ag13387
• Nom complet: melan-A
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: BC014423
• Symbole du gène: MelanA
• Identification du gène (NCBI): 2315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

Publications

Species	Application	Title
human	IF	Cancer Commun (Lond); Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.; Authors - Chaoji Shi
human	IF	Invest Ophthalmol Vis Sci; The Spatial Transcriptomic Atlas of Human Limbus and Vital Niche Microenvironment Regulating the Fate of Limbal Epithelial Stem Cells; Authors - Shiding Li
human	IF	Inflammation; Sirtuin1 Deficiency Could Exacerbate Melanocyte Apoptosis Under Endoplasmic Reticulum Stress; Authors - Jing Zhu

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