Anticorps Polyclonal de lapin anti-MLF1IP

• Phare
• Validé par KD/KO

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13186-1-PBS

Synonymes

CENP 50, CENP U, CENP U(50), CENP50, CENP-50

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Galerie de données de validation

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  WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13186-1-PBS cible MLF1IP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLF1IP Protéine recombinante Ag3858
• Nom complet: MLF1 interacting protein
• Masse moléculaire calculée: 418 aa, 48 kDa
• Poids moléculaire observé: 46-50 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC031520
• Symbole du gène: MLF1IP
• Identification du gène (NCBI): 79682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

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