Anticorps Polyclonal de lapin anti-MLH1

• Phare
• Validé par KD/KO

MLH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(35)

Avis (2)

N° de cat : 11697-1-AP

Synonymes

COCA2, FCC2, hMLH1, HNPCC, HNPCC2

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Galerie de données de validation

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  WB analysis of HeLa using 11697-1-AP

  WB result of MLH1 antibody (11697-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells.

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  WB analysis using 11697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11697-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11697-1-AP

  IP result of anti-MLH1 (IP:11697-1-AP, 4ug; Detection:11697-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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  IHC staining of human colon cancer using 11697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11697-1-AP (MLH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11697-1-AP (MLH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11697-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11697-1-AP (MLH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 13 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Informations sur le produit

11697-1-AP cible MLH1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLH1 Protéine recombinante Ag2319
• Nom complet: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Masse moléculaire calculée: 756 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC006850
• Symbole du gène: MLH1
• Identification du gène (NCBI): 4292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

Publications

Species	Application	Title
mouse	IHC	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
zebrafish	WB	Nucleic Acids Res; Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.; Authors - Jingzhen Li
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
human	WB	Nucleic Acids Res; Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.; Authors - Yi Yuan
human	IHC	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elena (Verified Customer) (06-26-2025)

Works for us for IP

• Applications: Immunoprecipitation

FH

Manohar (Verified Customer) (12-18-2021)

No non-specific binding, blocked with milk for 1 hour and antibody incubation at 4degrees over night

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

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