Anticorps Monoclonal anti-MLH1

MLH1 Monoclonal Antibody for IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D5A2

N° de cat : 60822-2-Ig

Synonymes

2D5A2, COCA2, FCC2, hMLH1, HNPCC

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Galerie de données de validation

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  IHC staining of human appendicitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60822-2-Ig cible MLH1 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MLH1 Protéine recombinante Ag27723
• Nom complet: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Masse moléculaire calculée: 756 aa, 85 kDa
• Numéro d’acquisition GenBank: BC006850
• Symbole du gène: MLH1
• Identification du gène (NCBI): 4292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer.

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