Anticorps Monoclonal anti-MLH1

• Phare
• Validé par KD/KO

MLH1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F12C4

N° de cat : 67350-1-PBS

Synonymes

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

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Galerie de données de validation

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  WB analysis using 67350-1-Ig (same clone as 67350-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67350-1-Ig (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67350-1-Ig (same clone as 67350-1-PBS)

  WB result of MLH1 antibody (67350-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67350-1-PBS cible MLH1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MLH1 Protéine recombinante Ag27723
• Nom complet: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Masse moléculaire calculée: 756 aa, 85 kDa
• Poids moléculaire observé: 85-100 kDa, 40-45 kDa
• Numéro d’acquisition GenBank: BC006850
• Symbole du gène: MLH1
• Identification du gène (NCBI): 4292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

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