Anticorps Recombinant de lapin anti-MLH1

MLH1 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241472D1

N° de cat : 84208-2-PBS

Synonymes

241472D1, COCA2, FCC2, hMLH1, HNPCC

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Galerie de données de validation

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  WB analysis using 84208-2-RR (same clone as 84208-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84208-2-RR (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

  IP result of anti-MLH1 (IP:84208-2-RR, 4ug; Detection:84208-2-RR 1:1000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84208-2-RR (same clone as 84208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84208-2-RR (MLH1 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84208-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84208-2-PBS against Human MLH1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 84208-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84208-2-RR against Human MLH1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84208-2-PBS cible MLH1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MLH1 Protéine recombinante Ag2319
• Nom complet: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Masse moléculaire calculée: 756 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC006850
• Symbole du gène: MLH1
• Identification du gène (NCBI): 4292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band.

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