Anticorps Polyclonal de lapin anti-MLK3

• Phare
• Validé par KD/KO

MLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11996-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11996-1-AP (same clone as 11996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11996-1-AP (same clone as 11996-1-PBS)

  WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 11996-1-AP (same clone as 11996-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11996-1-AP (same clone as 11996-1-PBS)

  Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11996-1-PBS cible MLK3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLK3 Protéine recombinante Ag2636
• Nom complet: mitogen-activated protein kinase kinase kinase 11
• Masse moléculaire calculée: 847 aa, 93 kDa
• Poids moléculaire observé: 93 and 65 kDa
• Numéro d’acquisition GenBank: BC011263
• Symbole du gène: MLK3
• Identification du gène (NCBI): 4296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).

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