Anticorps Polyclonal de lapin anti-MLKL

• Phare
• Validé par KD/KO

MLKL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(67)

N° de cat : 21066-1-AP

Synonymes

Mixed lineage kinase domain-like protein, hMLKL

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Galerie de données de validation

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  WB analysis using 21066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21066-1-AP (MLKL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21066-1-AP

  IP result of anti-MLKL (IP:21066-1-AP, 4ug; Detection:21066-1-AP 1:1000) with HepG2 cells lysate 1470 ug.

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  IHC staining of human liver cancer using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21066-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (21066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 60 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21066-1-AP cible MLKL dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLKL Protéine recombinante Ag15188
• Nom complet: mixed lineage kinase domain-like
• Masse moléculaire calculée: 471 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC028141
• Symbole du gène: MLKL
• Identification du gène (NCBI): 197259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.; Authors - Alex Rosenberg ; KO Validated
human	WB	Cancer Lett; Modulation of YBX1-mediated PANoptosis inhibition by PPM1B and USP10 confers chemoresistance to oxaliplatin in gastric cancer; Authors - Chunlin Lin
	WB	Front Immunol; N-acetyl-L-cysteine alleviated the oxidative stress-induced inflammation and necroptosis caused by excessive NiCl2 in primary spleen lymphocytes; Authors - Xintong Zhang
	WB,IF	Cell Death Discov; MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury; Authors - Jian Xu
human	WB	Cancers (Basel); STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.; Authors - Zhen-Yuan Zheng
	WB	Am J Chin Med; Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.; Authors - Xiaxia Wu

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