Anticorps Recombinant de lapin anti-MLL4

MLL4 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241819A4

N° de cat : 84410-1-PBS

Synonymes

241819A4, CXXC10 , DYT28, HRX2, KIAA0304

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Galerie de données de validation

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  WB analysis using 84410-1-RR (same clone as 84410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84410-1-RR (MLL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84410-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01298-1

  Sandwich ELISA standard curve of MP01298-1, Human MLL4 Recombinant Matched Antibody Pair - PBS only. 84410-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35385. 84410-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

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  Sample test of MP01298-1

  The mean MLL4 concentration was determined to be 1.5 ng/mL in HeLa cell extract based on a 1.8 mg/mL extract load.

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  Cytometric bead array standard curve of MP01298-1

  Cytometric bead array standard curve of MP01298-1, MLL4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84410-1-PBS. Detection antibody: 84410-2-PBS. Standard: Ag35385. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84410-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84410-1-RR against Human MLL4 were performed. The affinity constant is 22.6 pM.

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Informations sur le produit

84410-1-PBS cible MLL4 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MLL4 Protéine recombinante Ag35385
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia 4
• Poids moléculaire observé: 294 kDa
• Numéro d’acquisition GenBank: BC009337
• Symbole du gène: MLL4
• Identification du gène (NCBI): 9757
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating cleavage products.

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