Anticorps Polyclonal de lapin anti-MLL5

MLL5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14540-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HL-60 using 14540-1-AP (same clone as 14540-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  WB analysis using 14540-1-AP (same clone as 14540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14540-1-AP (same clone as 14540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14540-1-AP (same clone as 14540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 14540-1-AP (same clone as 14540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using MLL5 antibody (14540-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14540-1-PBS cible MLL5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLL5 Protéine recombinante Ag6044
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
• Masse moléculaire calculée: 205 kDa
• Poids moléculaire observé: 100 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC062583
• Symbole du gène: MLL5
• Identification du gène (NCBI): 55904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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