Anticorps Polyclonal de lapin anti-MLPH

• Phare
• Validé par KD/KO

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10338-1-PBS

Synonymes

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

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Galerie de données de validation

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  WB analysis using 10338-1-AP (same clone as 10338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10338-1-AP (MLPH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

  IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10338-1-AP (same clone as 10338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Melanophilin antibody (10338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10338-1-PBS cible MLPH dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLPH Protéine recombinante Ag0316
• Nom complet: melanophilin
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC001653
• Symbole du gène: MLPH
• Identification du gène (NCBI): 79083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

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