Anticorps Polyclonal de lapin anti-MLXIP

• Phare
• Validé par KD/KO

MLXIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13614-1-AP

Synonymes

bHLHe36, KIAA0867, MIR, MLX interacting protein, MLXIP, MONDOA

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Galerie de données de validation

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  WB analysis of K-562 using 13614-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13614-1-AP (MLXIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13614-1-AP

  IP Result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IP experiment of K-562 using 13614-1-AP

  IP Result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13614-1-AP

  Immunofluorescent analysis of HepG2 cells, using MLXIP antibody 13614-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

13614-1-AP cible MLXIP dans les applications de WB, IP, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLXIP Protéine recombinante Ag4519
• Nom complet: MLX interacting protein
• Masse moléculaire calculée: 919 aa, 100 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC039704
• Symbole du gène: MLXIP
• Identification du gène (NCBI): 22877
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Publications

Species	Application	Title
human	WB	Cancer Cell; Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.; Authors - Patrick A Carroll
mouse	chIP	Nat Neurosci; PMID: 31636448; Authors - Hei-Man Chow
mouse	WB,FC,ChIP	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	ChIP	Proc Natl Acad Sci U S A; Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.; Authors - Liangliang Shen
human	WB	Diabetes; MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.; Authors - Paul Richards
human	WB	PLoS Pathog; Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.; Authors - Erica L Sanchez