Anticorps Polyclonal de lapin anti-MLXIPL

MLXIPL Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13256-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

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  WB analysis using 13256-1-AP (same clone as 13256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13256-1-AP (same clone as 13256-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13256-1-AP (same clone as 13256-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  IP result of anti-MLXIPL (IP:13256-1-AP, 4ug; Detection:13256-1-AP 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13256-1-PBS cible MLXIPL dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLXIPL Protéine recombinante Ag3481
• Nom complet: MLX interacting protein-like
• Masse moléculaire calculée: 852 aa, 93 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC012925
• Symbole du gène: MLXIPL
• Identification du gène (NCBI): 51085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carbohydrate response element-binding protein (ChREBP), also known as MLXIPL, is a glucose-responsive transcription factor that has a fundamental role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization [PMID:21665952]. It enhances expression of the liver pyruvate kinase (LPK) gene and all lipogenic enzyme genes resulting in the conversion of excess dietary carbohydrate into triglycerides that can be stored as fat [PMID:15118080]. Molecular Weight of ChREBP splice variants: 62/78/91/93 kDa.

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