Anticorps Polyclonal de lapin anti-MMAB

MMAB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11137-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11137-1-AP (same clone as 11137-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 11137-1-AP (same clone as 11137-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 11137-1-AP (same clone as 11137-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11137-1-AP (same clone as 11137-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  IP result of anti-MMAB (IP:11137-1-AP, 4ug; Detection:11137-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11137-1-AP (same clone as 11137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11137-1-AP (MMAB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MMAB antibody (11137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11137-1-PBS cible MMAB dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMAB Protéine recombinante Ag1602
• Nom complet: methylmalonic aciduria (cobalamin deficiency) cblB type
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC011831
• Symbole du gène: MMAB
• Identification du gène (NCBI): 326625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

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