Anticorps Polyclonal de lapin anti-MMADHC

MMADHC Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC

Conjugaison

Non conjugué

N° de cat : 23191-1-AP

Synonymes

C2orf25, cblD, CL25022, MMADHC, My011

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Galerie de données de validation

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  WB analysis using 23191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23191-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23191-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules NIH3T3
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

23191-1-AP cible MMADHC dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMADHC Protéine recombinante Ag19343
• Nom complet: methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
• Masse moléculaire calculée: 296 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC022859
• Symbole du gène: MMADHC
• Identification du gène (NCBI): 27249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.

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