Anticorps Polyclonal de lapin anti-Neprilysin/CD10
Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 10302-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu rénal de souris, cellules HEK-293, tissu rénal de rat |
Résultats positifs en IHC | tissu rénal humain, tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu de cirrhose hépatique humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules MCF-7, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 2 publications below |
Informations sur le produit
10302-1-AP cible Neprilysin/CD10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris, Hamster |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Neprilysin/CD10 Protéine recombinante Ag0427 |
Nom complet | membrane metallo-endopeptidase |
Masse moléculaire calculée | 750 aa, 86 kDa |
Poids moléculaire observé | 90-100 kDa |
Numéro d’acquisition GenBank | BC101658 |
Symbole du gène | CD10 |
Identification du gène (NCBI) | 4311 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).
Protocole
Product Specific Protocols | |
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WB protocol for Neprilysin/CD10 antibody 10302-1-AP | Download protocol |
IHC protocol for Neprilysin/CD10 antibody 10302-1-AP | Download protocol |
IF protocol for Neprilysin/CD10 antibody 10302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Pathol Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion. | ||
Fitoterapia A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Luca (Verified Customer) (09-12-2022) | The antibody unfortunately did not work but most likely the conditions of the WB need optimized.
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FH Luca (Verified Customer) (06-10-2022) | I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.
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FH Boyan (Verified Customer) (10-27-2021) | This antibody recognised a clean band around 100 kd.
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