Anticorps Polyclonal de lapin anti-Neprilysin/CD10

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 10302-1-AP

Synonymes

MME, Neprilysin, Atriopeptidase, CALLA, CD10



Applications testées

Résultats positifs en WBtissu rénal de souris, cellules HEK-293, tissu rénal de rat
Résultats positifs en IHCtissu rénal humain, tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu de cirrhose hépatique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules MCF-7,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 2 publications below

Informations sur le produit

10302-1-AP cible Neprilysin/CD10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris, Hamster
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Neprilysin/CD10 Protéine recombinante Ag0427
Nom complet membrane metallo-endopeptidase
Masse moléculaire calculée 750 aa, 86 kDa
Poids moléculaire observé 90-100 kDa
Numéro d’acquisition GenBankBC101658
Symbole du gène CD10
Identification du gène (NCBI) 4311
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Protocole

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 10302-1-APDownload protocol
IHC protocol for Neprilysin/CD10 antibody 10302-1-APDownload protocol
IF protocol for Neprilysin/CD10 antibody 10302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Pathol

Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.

Authors - Hsiao-Fan Lo
human,hamsterWB

Fitoterapia

A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy

Authors - Saijuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Luca (Verified Customer) (09-12-2022)

The antibody unfortunately did not work but most likely the conditions of the WB need optimized.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Luca (Verified Customer) (06-10-2022)

I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500- 1:1000
FH

Boyan (Verified Customer) (10-27-2021)

This antibody recognised a clean band around 100 kd.

  • Applications: Western Blot
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