Anticorps Polyclonal de lapin anti-MME,CD10

MME,CD10 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (3)

N° de cat : 10302-1-AP

Synonymes

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

---

Galerie de données de validation

• 

  WB analysis using 10302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10302-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 10302-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (10302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal de rat
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10302-1-AP cible MME,CD10 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MME,CD10 Protéine recombinante Ag0427
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: MME
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publications

Species	Application	Title
mouse	WB	J Pathol; Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.; Authors - Hsiao-Fan Lo
human,hamster	WB	Fitoterapia; A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy; Authors - Saijuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Luca (Verified Customer) (09-12-2022)

The antibody unfortunately did not work but most likely the conditions of the WB need optimized.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Luca (Verified Customer) (06-10-2022)

I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500- 1:1000

FH

Boyan (Verified Customer) (10-27-2021)

This antibody recognised a clean band around 100 kd.

• Applications: Western Blot