Anticorps Polyclonal de lapin anti-MME,CD10

MME,CD10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 18008-1-AP

Synonymes

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie de données de validation

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  WB analysis using 18008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18008-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18008-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 18008-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 18008-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 18008-1-AP

  IP Result of anti-MME,CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.

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  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 18008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human kidney using 18008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Ramos using 18008-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (18008-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu rénal humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal humain,
• Résultats positifs en cytométrie: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

18008-1-AP cible MME,CD10 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MME,CD10 Protéine recombinante Ag12506
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 80aa,9 kDa; 750aa,85 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC106070
• Symbole du gène: MME
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human	WB	Int J Biol Macromol; A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.; Authors - Hui Zeng
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	IHC	BMC Genomics; Genetic control of renal tumorigenesis by the mouse Rtm1 locus.; Authors - Jensen José Ricardo JR
mouse	WB	J Alzheimers Dis; Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.; Authors - Ling-Li Lv

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

• Applications: Western Blot

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C