Anticorps Monoclonal anti-MMP-9

MMP-9 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3C3E2

N° de cat : 60600-1-Ig

Synonymes

MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35

---

Galerie de données de validation

• 

  WB analysis using 60600-1-Ig

  Supernatant from non-treated and PMA treated U2OS cells were subjected to SDS PAGE followed by western blot with 60600-1-Ig (MMP-9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60600-1-Ig cible MMP-9 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP-9 Protéine recombinante Eg0442
• Nom complet: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Masse moléculaire calculée: 707 aa, 78 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC006093
• Symbole du gène: MMP-9
• Identification du gène (NCBI): 4318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling (PMID:7492685).

Publications

Species	Application	Title
mouse	WB	Aging Dis; ATF4 Contributes to Abdominal Aortic Aneurysm Formation via Modulating M1 Macrophage Polarization and Inflammation; Authors - Huahui Yu

{{ptg:RelatedPrimaryAntibodies}}