Anticorps Monoclonal anti-MMP-9

MMP-9 Monoclonal Antibody for WB, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

3C3E2

N° de cat : 60600-1-PBS

Synonymes

MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35

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Galerie de données de validation

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  WB analysis using 60600-1-Ig (same clone as 60600-1-PBS)

  Supernatant from non-treated and PMA treated U2OS cells were subjected to SDS PAGE followed by western blot with 60600-1-Ig (MMP-9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60600-1-PBS in a different storage buffer formulation.

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  Sample test of MP51000-1

  Cytometric bead array of A549 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. A549 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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  Sample test of MP51000-1

  Cytometric bead array of MM231 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. MM231 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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  Sample test of MP51000-1

  Cytometric bead array of U2OS cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. U2OS cells were cultured for 2 days in normal culture media or PMA (200nM, 48h) containing media. 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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Informations sur le produit

60600-1-PBS cible MMP-9 dans les applications de WB, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP-9 Protéine recombinante Eg0442
• Nom complet: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Masse moléculaire calculée: 707 aa, 78 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC006093
• Symbole du gène: MMP-9
• Identification du gène (NCBI): 4318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling (PMID:7492685).

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