Anticorps Polyclonal de lapin anti-MMP1

• Phare
• Validé par KD/KO

MMP1 Polyclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

166

Avis (1)

N° de cat : 10371-2-AP

Synonymes

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1

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Galerie de données de validation

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  WB analysis of U-87 MG using 10371-2-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 10371-2-AP (MMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 cells using 10371-2-AP

  WB results of MMP1 antibody (10371-1-AP, 1:500) with NIH3T3 cells, 62 kDa for pro-MMP1 and 54 kDa for Active MMP1. Data from Dr. Hui-Wen Chiu, Taipei Medical University.

• 

  WB analysis using 10371-2-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 10371-2-AP (MMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 10371-2-AP

  Immunofluorescent analysis of HepG2 cells, using MMP1 antibody 10371-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 10371-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug MMP1 antibody (10371-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules NIH/3T3
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 139 publications below
• IHC: See 30 publications below
• IF: See 3 publications below

Informations sur le produit

10371-2-AP cible MMP1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP1 Protéine recombinante Ag0547
• Nom complet: matrix metallopeptidase 1 (interstitial collagenase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52-56 kDa, 62 kDa
• Numéro d’acquisition GenBank: BC013875
• Symbole du gène: MMP1
• Identification du gène (NCBI): 4312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Publications

Species	Application	Title
human	WB	Cell Res; Identification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma.; Authors - Fei Li
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
rat	WB	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang
human	IHC	Br J Cancer; Chemerin suppresses hepatocellular carcinoma metastasis through CMKLR1-PTEN-Akt axis.; Authors - Jing-Jing Li
rat	WB,IF	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	WB	Cancer Lett; Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.; Authors - Jiao Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Saravanakumar (Verified Customer) (10-31-2017)

I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A cells

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