Anticorps Recombinant de lapin anti-MMP1

MMP1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230343B6

N° de cat : 83114-3-PBS

Synonymes

22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7

---

Galerie de données de validation

• 

  Sandwich ELISA standard curve of MP00290-1

  Sandwich ELISA standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. 83114-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0527. 83114-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

• 

  Sample test of MP00290-1

  Plasma of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 653.4 pg/mL with a range of 99.3 - 1,631.8 pg/mL.

• 

  Sample test of MP00290-1

  HT1080 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MMP1, and measured 907.5 pg/mL.

• 

  Sample test of MP00290-1

  Serum of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 9,124.3 pg/mL with a range of 3,045.5 - 19,864.3 pg/mL.

• 

  Cytometric bead array standard curve of MP00290-1

  Cytometric bead array standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83114-4-PBS. Detection antibody: 83114-3-PBS. Standard: Eg0527. Range: 0.625-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 83114-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83114-3-PBS against Human MMP1 was performed. The affinity constant is 12.7 nM.

---

Informations sur le produit

83114-3-PBS cible MMP1 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MMP1 Protéine recombinante Eg0527
• Nom complet: matrix metallopeptidase 1 (interstitial collagenase)
• Masse moléculaire calculée: 54 kDa
• Numéro d’acquisition GenBank: BC013875
• Symbole du gène: MMP1
• Identification du gène (NCBI): 4312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}