Anticorps Monoclonal anti-MMP12

• Phare
• Validé par KD/KO

MMP12 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1C6B5

N° de cat : 66930-1-Ig

Synonymes

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

---

Galerie de données de validation

• 

  WB analysis of A549 using 66930-1-Ig

  WB result of MMP12 antibody (66930-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP12 transfected A549 cells.

• 

  WB analysis using 66930-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66930-1-Ig (MMP12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules NCI-H1299

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

66930-1-Ig cible MMP12 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP12 Protéine recombinante Ag19068
• Nom complet: matrix metallopeptidase 12 (macrophage elastase)
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 54 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC112301
• Symbole du gène: MMP12
• Identification du gène (NCBI): 4321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).

Publications

Species	Application	Title
mouse	IHC	Eur J Pharmacol; Reversal of elastase-induced abdominal aortic aneurysm following the delivery of nanoparticle-based pentagalloyl glucose (PGG) is associated with reduced inflammatory and immune markers.; Authors - Saphala Dhital
mouse	WB	Drug Des Devel Ther; Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification; Authors - Wenwen Chen
human,mouse	IHC	Cytokine; IL-33/ST2 enhances MMP-12 expression by macrophages to mediate inflammatory and immune response in IgG4-Related Ophthalmic Disease; Authors - Xia Ding
human	WB,IHC	Curr Eye Res; Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence; Authors - Shichao Han

{{ptg:RelatedPrimaryAntibodies}}