Anticorps Monoclonal anti-MMP12

• Phare
• Validé par KD/KO

MMP12 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C6B5

N° de cat : 66930-1-PBS

Synonymes

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

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Galerie de données de validation

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  WB analysis using 66930-1-Ig (same clone as 66930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66930-1-Ig (MMP12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66930-1-Ig (same clone as 66930-1-PBS)

  WB result of MMP12 antibody (66930-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP12 transfected A549 cells. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66930-1-PBS cible MMP12 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP12 Protéine recombinante Ag19068
• Nom complet: matrix metallopeptidase 12 (macrophage elastase)
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 54 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC112301
• Symbole du gène: MMP12
• Identification du gène (NCBI): 4321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).

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