Anticorps Recombinant de lapin anti-MMP2

MMP2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240131F5

N° de cat : 81515-2-PBS

Synonymes

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

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Galerie de données de validation

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  IF Staining of HepG2 using 81515-2-RR (same clone as 81515-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (81515-2-RR, Clone: 240131F5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81515-2-RR (same clone as 81515-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP2 Recombinant antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 81515-2-RR (same clone as 81515-2-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug MMP2 Recombinant Antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81515-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81515-2-RR against Human MMP2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81515-2-PBS cible MMP2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MMP2 Protéine recombinante Ag0549
• Nom complet: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 62-72 kDa
• Numéro d’acquisition GenBank: BC002576
• Symbole du gène: MMP2
• Identification du gène (NCBI): 4313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

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